A Three-Point Directional Search Block Matching Algorithm

This paper proposes compact directional asymmetric search patterns, which we have named as three-point directional search (TDS). In most fast search motion estimation algorithms, a symmetric search pattern is usually set at the minimum block distortion point at each step of the search. The design of the symmetrical pattern in these algorithms relies primarily on the assumption that the direction of convergence is equally alike in each direction with respect to the search center. Therefore, the monotonic property of real-world video sequences is not properly used by these algorithms. The strategy of TDS is to keep searching for the minimum block distortion point in the most probable directions, unlike the previous fast search motion estimation algorithms where all the directions are checked. Therefore, the proposed method significantly reduces the number of search points for locating a motion vector. Compared to conventional fast algorithms, the proposed method has the fastest search speed and most satisfactory PSNR values for all test sequences

DNA Binding and Photocleavage Studies of Cobalt(III) Ethylenediamine Pyridine Complexes: [Co(en)2(py)2]3+ and [Co(en)2(mepy)2]3+

Two novel cobalt(III) pyridine complexes (1) [Co(en)2(py)2]3+ and (2) [Co(en)2(mepy)2]3+ (en=ethylenediamine, py=pyridine, and mepy=methylpyridine) have been synthesized and characterized. The interaction of these complexes with calf thymus DNA was investigated by absorption, emission spectroscopy, viscosity measurements, DNA melting, and DNA photocleavage. Results suggest that the two complexes bind to DNA via groove mode and complex 2 binds more strongly to CT DNA than complex 1. Moreover, these Co(III) complexes have been found to promote the photocleavage of plasmid DNA pBR322 under irradiation at 365 nm, cytotoxicity results of complexes are also showing anticancer activity

Student Monitering using An Embedded Real Time Finger-Vein Recognition System

Abstract: In the academic institutions especially in professional institutions, attendance is a very important criterion which is used for various purposes. But, at the same time attendance status of the students should also be important to their Parent/Guardian, because of the mode of travel they use, which may be own/public vehicle and of course sometimes fraudulence by the words, becomes essential to track their ward. In the present traditional method, attendance involves the use of sheets of paper or books in taking student attendance, leads towards various challenges like time consuming, complexity of the calculations, occurrence of human errors, and could be stolen, damaged or lost. Thus, there is a need for a system that would eliminate all of these trouble spots. Our finger print recognition system would provide the needed solution. In this attendance management system Rather answering for attendance individual has to pass his/her thumb over the fingerprint scanner and the fingerprint is compared against a list of preregistered users, and once a match are made, the individual will be registered. As soon as the attendance of a student is registered, a message would be sent to his/her Parent/Guardian. at the moment itself about his presence in the class, which makes them the avoidance of tracking or worrying about their ward. And also we are adding time table alert system for department. Here we design an embedded system to store the time table of a class and send an alert message to the faculty just few minutes before the commencement of the class

Depolymerization of phospholamban in the presence of calcium pump: a fluorescence energy transfer study. Biochem

cardiac sarcoplasmic reticulum (SR) through PLB phosphorylation mediated by �-adrenergic stimulation. The mobility of PLB on SDS-PAGE indicates a homopentamer, and it has been proposed that the pentameric structure of PLB is important for its regulatory function. However, the oligomeric structure of PLB must be determined in its native milieu, a lipid bilayer containing the Ca-ATPase. Here we have used fluorescence energy transfer (FET) to study the oligomeric structure of PLB in SDS and dioleoylphosphatidylcholine (DOPC) lipid bilayers reconstituted in the absence and presence of Ca-ATPase. PLB was labeled, specifically at Lys 3 in the cytoplasmic domain, with amine-reactive fluorescent donor/ acceptor pairs. FET between donor- and acceptor-labeled subunits of PLB in SDS solution and DOPC lipid bilayers indicated the presence of PLB oligomers. The dependence of FET efficiency on the fraction of acceptor-labeled PLB in DOPC bilayers indicated that it is predominantly an oligomer having 9-11 subunits, with ∼10 % of the PLB as monomer, and the distance between dyes on adjacent PLB subunits is 0.9 ( 0.1 nm. When labeled PLB was reconstituted with purified Ca-ATPase, FET indicated the depolymerization of PLB into smaller oligomers having an average of 5 subunits, with a concomitant increase in the fraction of monomer to 30-40 % and a doubling of the intersubunit distance. We conclude that PLB exists primarily as an oligomer in membranes, and the Ca-ATPase affects the structure of thi

Co-reconstitution of Phospholamban Mutants with the Ca-ATPase Reveals Dependence of Inhibitory Function on Phospholamban Structure*

in cardiac sarcoplasmic reticulum through PLB phosphorylation mediated by �-adrenergic stimulation. Based on site-directed mutagenesis and coexpression with Ca-ATPase (SERCA2a) in Sf21 insect cells or in HEK 293 cells, and on spin label detection of PLB oligomeric state in lipid bilayers, it has been proposed that the monomeric form of PLB is the inhibitory species, and depolymerization of PLB is essential for its regulatory function. Here we have studied the relationship between PLB oligomeric state and function by in vitro co-reconstitution of PLB and its mutants with purified Ca-ATPase. We compared wild type-PLB (wt-PLB), which is primarily a pentamer on SDS-polyacrylamide gel electrophoresis (PAGE) at 25 °C, with two of its mutants, C41L-PLB and L37A-PLB, that are primarily tetrame